BIOPHEN™ Heparin (LRT)
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BIOPHEN™ Heparin (LRT) (221013) is an Anti-Xa chromogenic method for the assay of Heparin and their analogs, and direct Factor Xa (FXa) inhibitors, with ready to use liquid reagents. Manufactured by HYPHEN BioMed.
The BIOPHEN™ Heparin LRT method is a one stage chromogenic assay based on the inhibition of a constant amount and in excess of FXa, by heparin (or other anti-Xa substance) to be assayed, in the presence of endogenous AT. The residual FXa hydrolyses a specific chromogenic substrate (SXa-11) releasing paranitroaniline (pNA)3 . The quantity of released pNA (measured by absorbance at 405 nm) is inversely proportional to the concentration of heparin (or other anti-Xa substance) present in the reaction medium. Heparin and analogues Heparin + AT ï¨ [AT Hep.] [AT Hep.] + [FXa (excess)] ï¨ [FXa-AT-Hep.] + [residual FXa] [FXa (residual)] + SXa-11 ï¨ Peptide + pNA Rivaroxaban / Apixaban / Edoxaban (DiXaI) [DiXaI] + [FXa (excess)] ï¨ [FXa-DiXaI] + [FXa residual] [FXa (residual)] + Substrate ï¨ Peptide + pNA