BIOPHEN™ Heparin Anti-IIa (kinetics)
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BIOPHEN™ Heparin Anti-IIa (kinetics) (221020) is a Kinetics/competitive method for the measurement of Heparin, and Heparin-like anticoagulants, in buffer, using an anti-IIa Chromogenic Assay FOR RESEARCH USE ONLY. Manufactured by HYPHEN BioMed.
The Heparin Anti-IIa method is a kinetics/competitive chromogenic anti-IIa assay developed for measuring heparin (UFH), in buffers or purified milieu. Heparin is a sulphated polysaccharide with a high affinity for antithrombin. When complexed with heparin, antithrombin exhibits a fast acting and potent inhibitory activity for coagulant serine esterases: IXa, Xa and thrombin. LMWH, and heparin analogues, such as Sodium DanaparoÃ¯d, inhibit more efficiently Factor Xa than thrombin. Anti-IIa assays are then the right methods for measuring the anti-thrombin activity of large heparin molecules. The Heparin Anti-IIa method is a kinetics/competitive method based on the inhibition of a constant amount of Thrombin (IIa), by the tested heparin in presence of exogenous antithrombin, and the simultaneous hydrolysis of a Thrombin specific chromogenic substrate, by remaining active Thrombin. pNA is then released from the substrate. The amount of pNA released is then a relation of the residual Thrombin activity. There is an inverse relationship between the concentration of heparin and color development, measured at 405 nm. Heparin + AT ï¨[AT Hep.] [AT Hep.] + [IIa (excess)] ï¨ [FIIa-AT-Hep.] + [residual FIIa] [FIIa (residual)] + IIa-Subs. ï¨ Peptide + pNA