BIOPHEN™ Heparin 3
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BIOPHEN™ Heparin 3 (221003) is a measurement of Heparin, and Heparin like anticoagulants, using an anti-Xa chromogenic method. Manufactured by HYPHEN BioMed.
The BIOPHEN Heparin 3 assay is a kinetic method based on the inhibition of a constant and in excess amount of Factor Xa, by heparin (or other anti-Xa) to be assayed, in the presence of endogenous antithrombin. The residual factor Xa hydrolysis a specific chromogenic substrate (SXa-11) releasing paranitroaniline (pNA)3 . The quantity of released pNA (measured by absorbance at 405 nm) is inversely proportional to the concentration of heparin (or other anti-Xa substance) present in the medium reaction. Heparin + AT ï¨ [AT Hep.] [AT Hep.] + [FXa (excess)] ï¨ [FXa-AT-Hep.] + [FXa (residual)] [FXa (residual)] + SXa-11 ï¨ Peptide + pNA