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BIOPHEN™ Heparin (AT+)


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BIOPHEN™ Heparin (AT+) (221007) is a measurement of Heparin, and Heparin-like anticoagulants, using an antiXa kinetics chromogenic method, with addition of exogenous ATIII FOR RESEARCH USE ONLY. Manufactured by HYPHEN BioMed.

Product Brand:
Product Code: 221007

Biophen Heparin (AT+) is a kinetics chromogenic anti-Xa method developed for measuring homogeneously heparin (UFH) and Low Molecular Weight Heparin (LMWH), using the same calibration curve, provided that the adaptation used allows this superimposition. Heparin is a sulphated polysaccharide with a high affinity for antithrombin. When complexed with heparin, antithrombin exhibits a fast acting and potent inhibitory activity for coagulant serine esterases: IXa, Xa and thrombin. LMWH, and heparin analogues, such as Sodium Danaparoïd, inhibit more efficiently Factor Xa than thrombin. Anti-Xa assays are then the methods of choice for measuring heparins and their analogues. Biophen Heparin (AT+) is a kinetics method based on the inhibition of a constant amount of factor Xa, by the tested heparin in presence of exogenous antithrombin, and hydrolysis of a Factor Xa specific chromogenic substrate (SXa-11), by the factor Xa in excess. pNA is then released from the substrate. The amount of pNA released is then a relation of the residual factor Xa activity. There is an inverse relationship between the concentration of heparin and color development, measured at 405 nm.

Product Specification
Specification Type
Specification Value
Product Range
Product Code
Product Name
BIOPHEN™ Heparin (AT+)
Product Category
Product Brand
Product Analyte or Application
Product Size
2 x 7.5 ml
Product Storage
2°C to 8°C

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