Rox Tris-10% BSA 50 mL
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Rox Tris-10% BSA 50 mL (TB035-50) is a diluent buffer, stock solution, for sensitive proteins such as coagulation factors. Diluted buffer fulfils the Ph Eur requirements on a coagulation Factor VIII diluent. Manufactured by Rossix AB.
Key characteristics
- Tris BSA Stock solution – To be diluted 1:10 with water prior to use.
- Stock solution: 0.5 M Tris pH 7.3 (20°C) 2.0 M NaCl 10% BSA.
- Contains carefully validated Bovine Serum Albumin (BSA) with Australian or New Zeeland origin.
- 1:10 dilution in water fulfils the Ph Eur requirements on a coagulation Factor VIII diluent.
- Shelf life 3.5 years at 2-8°C.
Measurement principle
FIX activity is determined in a chromogenic method, in which human FIX is activated by human FXIa and where formed FIXa activates human FX in the presence of human FVIII, calcium ions and phospholipid. Similar to in vivo conditions, FVIII is activated by thrombin which is generated during the incubation. The amount of FXa formed is related to the FIX activity and is determined from the hydrolysis of a chromogenic FXa substrate. The FIX activity of the sample is assigned vs. a FIX plasma or a FIX concentrate standard with FIX potency expressed in International Units (IU).
Kit information
- Available as 50 mL (TB035) or 100 mL (TB035-100) per vial.
- For In Vitro Research Use Only.
