Filamentous Hemagglutinin (FHA) from Bordetella Pertussis (Special Terms Apply*)

Product Overview:

Filamentous Hemagglutinin (FHA) from Bordetella pertussis is a purified protein preparation intended for in vitro research applications. This product is supplied in liquid form, with each container holding 25 μg of protein in 0.05 M sodium phosphate and 0.5 M sodium chloride (pH 7.0), at a final concentration of 0.1 mg/mL. It is designed for use by qualified personnel in a laboratory setting. Research use only reagent.

Key Features and Benefits:

• Defined Concentration: Provided at a ready-to-use concentration of 0.1 mg/mL for consistent and reproducible experimental use.
• Purity Assessed: Characterised with a purity of 70% as determined by both reduced and non-reduced SDS-PAGE.
• Low Endotoxin Levels: Specified at 9.9 EU/mg, making it suitable for research applications where endotoxin presence is a concern.
• Functionally Active: Product activity is confirmed using a hemagglutination assay, with a positive response observed at 0.0006 mg/mL.

Principle and Application:

This product is a purified protein from Bordetella pertussis. As a key virulence factor, FHA is a common subject of research in areas such as bacterial pathogenesis, vaccine development, and cell adhesion. This reagent can be used as a standard, in immunoassays, or for cellular studies, though the specific application and methodology must be determined by the end-user.

Product Composition and Specifications:

This product is a liquid reagent, provided clear, colorless, and free of particulates.

• Fill Volume: 0.25 mL
• Total Protein: 25 μg per container
• Buffer: 0.05 M sodium phosphate, 0.5 M sodium chloride, pH 7.0
• Concentration: 0.1 mg/mL
• OD Measurement: A₂₈₀/A₂₆₀: 0.85; A₃₂₀: 0.09
• Purity (SDS-PAGE): 70% (Non-reduced); 70% (Reduced)
• Endotoxin: 9.9 EU/mg

Performance Characteristics:

• Activity (Hemagglutination Assay): The lowest concentration showing a positive response for agglutination using sheep red blood cells was 0.0006 mg/mL.

Storage and Stability:

• Unopened Vials: Store at ≤-65°C. Ship on dry ice.
• Handling: Handle the product gently; do not vortex.

Disclaimer:

The information on this page is for informational purposes and serves as a general summary. It is imperative that all users read and strictly follow the Instructions for Use (IFU) document provided with the reagent or kit they are using. This online description must not be a substitute for the official product documentation.