Easy Reader+® Calibrator card
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Easy Reader+® Calibrator card (C.CARD) is a calibrator card for use with the Easy Reader+®. Manufactured by VEDALAB.
The EasyReader+® instrument is a quantitative lateral flow immunochromatography analyzer. The EasyReader+® installation kit includes the analyzer and the software that lets the operator actuate the evaluation processes, regulate the functioning, storing and moving measurement results database.
The EasyReader+® analyzer is intended to provide a quantitative immunochromatography assay result for a wide range of otherwise purely qualitative rapid test devices to increase the accuracy and efficiency of the home, point of care, and laboratory testing of the relevant marker analytes.
Rapid test device technology
The test specimen (type of sample that can be used on the instrument, as indicated in the corresponding device manual for each parameter) that the operator dispenses into the assay device migrates, with spontaneous capillary action, all through the membrane, and interacts with the particles and molecules placed at various parts of the membrane.
1 It first interacts with the conjugate, where it binds with the color-coated bio-active particles. The specimen analytes that pass through the conjugate therefore become color-coated themselves.
2 The specimen then passes through the Test Line (T) and the Control Line (C), where two types of capturing components are present in the membrane:
– The components in the Control Line capture every colored particle that passes through them, and indicate whether the assay device is functional: When an adequate number of colored particles, are captured, a colored band appears across the Control Line, which means that the test result is valid.
– The components in the Test Line are analyte-specific, and only capture test particles that bound to the analyte that the test detects. When an adequate number of analyte-bound colored particles are captured, a colored band appears across the Test Line, which indicates a positive test result.
Assay device processing
1 After the specimen is dispensed into the assay device, a device-specific incubation time must elapse before a reading can be made. You can time the incubation time yourself, or set the EasyReader+® device to count it down.
2 When the incubation time elapses, the instrument shines a green LED light onto the surface of the membrane of the assay device. The green light is used for evaluation and serves to increase the contrast of the potential discoloration by effectively subtracting the white background from the image.
3 A CMOS camera takes a picture of the oval test window of the device, using the light from the LEDs. The image processing software scans the resulting image, detects any discoloration, and calculates the average saturation of the colored area on the membrane.
4 The microprocessor compares the average saturation value detected in the image to the preset device-specific cutoff ranges to arrive at a semi-quantitative test result.