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Trichinella Spiralis

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Trichinella Spiralis (9750) is an ELISA kit for the diagnosis of human trichinellosis. Manufactured by Bordier Affinity Products.

Product Code: 9750
Description

Intended Use:
The Bordier Trichinella spiralis ELISA kit is intended for the quantitative detection of IgG antibodies against Trichinella spiralis in human serum. Serology is an aid for diagnosis and cannot be used as the sole method of diagnosis.


Background:

Trichinellosis is a worldwide zoonosis caused by Trichinella spiralis, a parasitic nematode of pork, horse and wild animals. Humans can be infected by eating raw or undercooked meat from infected animals. Outbreaks occur in settings where multiple people consume the same Trichinella-infected meat. Larvae are released from cysts by gastric exposition and invade the small intestinal mucosa. In this tissue, larvae develop into adult worms. In the intestinal lumen, female worms release “new-borne larvae” that migrate to the striated muscles where they encyst upon entering muscle cells. Most infected people do not show any symptoms. However, in some cases, symptoms appear during the intestinal stage (diarrhea and abdominal pain) and muscular stage (muscle pain, fever, edema). Diagnosis is based on signs and symptoms plus a history of exposure and a positive result by serological testing.


Principle and Presentation:
The kit provides all the material needed to perform 96 enzyme-linked immunosorbent assays (ELISA) on breakable microtitration wells sensitized with Trichinella spiralis excreted/secreted (E/S) larval antigens. Specific antibodies in the sample will bind to these antigens and washing will remove unspecific antibodies. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of pNPP substrate which turns yellow in the presence of alkaline phosphatase. Color intensity is proportional to the amount of Trichinella spiralis specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader.

The test can be performed with automatic systems, but this must be validated by the user.

Product Specification
Specification Type
Specification Value
Product Range
Product Code
9750
Product Name
Trichinella Spiralis
Product Category
Product Brand
Product Analyte or Application
Product Size
Kit of 96 tests
Product Storage
2°C to 8°C

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