Trichinella Spiralis ELISA Kit
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The Bordier Trichinella Spiralis ELISA Kit (9750) is an enzyme immunoassay for the diagnosis of human trichinellosis. Manufactured by Bordier Affinity Products.
The Bordier Trichinella spiralis ELISA kit is intended for the quantitative detection of IgG antibodies against Trichinella spiralis in human serum. Serology is an aid for diagnosis and cannot be used as the sole method of diagnosis.
Trichinellosis is a worldwide zoonosis caused by Trichinella spiralis, a parasitic nematode of pork, horse and wild animals. Humans can be infected by eating raw or undercooked meat from infected animals. Outbreaks occur in settings where multiple people consume the same Trichinella-infected meat. Larvae are released from cysts by gastric exposition and invade the small intestinal mucosa. In this tissue, larvae develop into adult worms. In the intestinal lumen, female worms release “new-borne larvae” that migrate to the striated muscles where they encyst upon entering muscle cells. Most infected people do not show any symptoms. However, in some cases, symptoms appear during the intestinal stage (diarrhea and abdominal pain) and muscular stage (muscle pain, fever, edema). Diagnosis is based on signs and symptoms plus a history of exposure and a positive result by serological testing.
Principle and Presentation:
The kit provides all the material needed to perform 96 enzyme-linked immunosorbent assays (ELISA) on breakable microtitration wells sensitized with Trichinella spiralis excreted/secreted (E/S) larval antigens. Specific antibodies in the sample will bind to these antigens and washing will remove unspecific antibodies. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of pNPP substrate which turns yellow in the presence of alkaline phosphatase. Color intensity is proportional to the amount of Trichinella spiralis specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader.
The test can be performed with automatic systems, but this must be validated by the user.
- Are Bordier kits CE-marked?
Yes. The Bordier kits are CE-marked for Medical Professional Use only in accordance with EU IVD regulations (IVDD 98/79/EC).
- Do Bordier hold ISO accreditation?
- Which components can be used with all Bordier ELISA kits?
- Can Bordier ELISA kits be automated?
- Is it be possible to order single components?
- Can Bordier ELISA kits be used with plasma?
- Can Bordier ELISA kits be used with animal serum?
- Can Bordier ELISA kits be used with other samples (cerebrospinal fluid, bronchoalveolar lavage, aqueous humor…)?
- Is a positive control provided with IFAT kits?