FIBRIPHEN™ 1
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FIBRIPHEN™ 1 (CK571K) is a Thrombin reagent for the quantitative determination of Fibrinogen in human citrated plasma using von Clauss clotting method. Manufactured by HYPHEN BioMed.
Intended Use
The FIBRIPHEN™ kit is a clotting method for in vitro quantitative determination of Fibrinogen in human citrated plasma (Clauss method), using manual or automated method.
Summary and Explanation
Technical: Fibrinogen is a 340 Kd soluble plasma glycoprotein, synthesized in the liver, containing 6 peptidic chains, with a 2 to 2 symmetry, and linked by disulfide bridges (2 Aα, 2 Bβ and 2 γ chains). Thrombin clots fibrinogen and forms fibrin, which is then stabilized by activated Factor XIII in presence of calcium. Fibrinogen is lysed by plasmin to fragments X and Y, first, then D and E.
Clinical: Fibrinogen concentration in normal human plasma is usually in the range 2 to 4 g/L. Elevated fibrinogen concentrations (> 4g/L) are observed in clinical situations associated with inflammation and have also been considered as a risk factor for cardiovascular disease and thrombosis. Hypofibrinogenemia is mainly associated with severe liver disease, or excessive consumption of fibrinogen (DIC, hyperfibrinolysis). Numerous variants of fibrinogen have been described, associated to asymptomatic cases, or to cases with bleeding and/or thrombosis.
Principle
In the presence of a constant and in excess amount of thrombin, the clotting time (CT) obtained for diluted citrated plasma depends on the plasma fibrinogen concentration.
Reagents
R Calcium Thrombin, from bovine origin (about 100 NIH/mL), lyophilized. Contains BSA, an heparin neutralizing substance and stabilizers.
FIBRIPHEN™ 1 REF CK571K ➔ 6 vials of 1 mL.
FIBRIPHEN™ 2 REF CK572K ➔ 6 vials of 2 mL.
FIBRIPHEN™ 5 REF CK575K ➔ 8 vials of 5 mL.
UKCA
