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BIOPHEN™ FVIII :C

X

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BIOPHEN™ FVIII :C (221402) is a Chromogenic Assay for measuring Factor VIII:C (FVIII:C) in plasma, or in concentrates. In vitro diagnostic use only. Manufactured by HYPHEN BioMed.

Product Brand:
Product Code: 221402
Description

When activated by thrombin, Factor VIII:C forms an enzymatic complex with Factor IXa, phospholipids and Calcium, which activates Factor X to Factor Xa. BIOPHEN Factor VIII:C is a chromogenic assay for testing the cofactor activity of Factor VIII:C. In presence of a constant amount of Factor IXa, Phospholipids (PLPs) and Calcium, thrombin activated Factor VIII:C forms an enzymatic complex, which activates Factor X, supplied in the assay at a constant concentration and in excess, to Factor Xa. This activity is directly related to the amount of Factor VIII:C, which is the limiting factor in presence of a constant and in excess amount of Factor IXa. Generated Factor Xa is then exactly measured by its activity on a specific Factor Xa chromogenic substrate (SXa-11). Factor Xa cleaves the substrate and releases pNA. The amount of pNA generated is directly proportional to the Factor Xa activity. Finally, there is a direct relationship between the amount of Factor VIII:C in the assayed sample and the Factor Xa activity generated, measured by the amount of pNA released, determined by color development at 405nm.

Product Specification
Specification Type
Specification Value
Product Range
Product Code
221402
Product Name
BIOPHEN™ FVIII :C
Product Category
Product Brand
Product Analyte or Application
Product Size
2 x 2.5 ml
Product Storage
2°C to 8°C

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