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BIOPHEN™ FVII (221304) is a Chromogenic Assay for quantitative determination of Factor VII (FVII) FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Manufactured by HYPHEN BioMed.
In the presence of tissue factor (rabbit thromboplastin) and calcium, Factor FVII forms an enzymatic complex which activates the Factor X, present at a constant concentration and in excess, to Factor Xa. The amount of Factor Xa formed depends on the concentration of Factor VII to be assayed. This Factor Xa formed cleaves the specific substrate Xa (SXa-11) and releases pNA. The amount of pNA generated is directly proportional to the Factor Xa activity. The amount of Factor VII in the assayed sample is directly proportional to the Factor Xa activity generated, measured by the amount of pNA released, determined by color development at 405 nm.