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BIOPHEN™ DiXaI (221030) is a Chromogenic method for the assay of direct Factor Xa (FXa) inhibitors (DiXaIs). Manufactured by HYPHEN BioMed.
BIOPHEN™ DiXal is a chromogenic method based on the inhibition, by the DiXal being assayed, of a constant and excess quantity of Factor Xa (FXa). The residual Factor Xa hydrolyses the FXa-specific chromogenic substrate, releasing paranitroaniline (pNa). The amount of pNa released (measured by absorbance at 405 nm) is inversely proportional to the concentration of DiXal in the sample. [DiXaI] + [FXa (excess)] ï¨ [FXa-DiXaI] + [FXa residual] [FXa (residual)] + Substrate ï¨ Peptide + pNA