BIOPHEN™ AT (Anti-IIa)
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BIOPHEN™ AT (Anti-IIa) (221122) is a Chromogenic method for quantitative determination of antithrombin (AT) activity in human plasma. Manufactured by HYPHEN BioMed.
Antithrombin (AT) is the major physiological coagulation inhibitor. It inhibits coagulation serine esterases, especially Thrombin, Factor Xa and Factor IXa, regulates coagulation pathway and prevents from thrombosis. When complexed to heparin, AT becomes a potent and fast acting inhibitor of coagulation serine esterases. This anti-IIa assay thus measures the heparindependent anti-IIa activity of AT2,3,4,5 . The BIOPHEN™ AT (Anti-IIa) method is an assay based on the inhibition of a constant and in excess amount of Thrombin (IIa), by the tested AT in presence of an excess of heparin, and hydrolysis of a Thrombin specific chromogenic substrate, by Thrombin in excess. pNA is then released from the substrate. The amount of pNA released (measured by absorbance at 405 nm) is then a relation of the residual Thrombin quantity. There is an inverse relationship to the concentration of AT present in the tested sample. Heparin + AT ï¨[AT Hep.] [AT Hep.] + [IIa (excess)] ï¨ [IIa-AT-Hep.] + [residual FIIa] [FIIa (residual)] + IIa-Subs. ï¨ Peptide + pNA