Check4® Myoglobin is a rapid qualitative test assay for the detection of human myoglobin, which can be used with diluted urine.
The method employs a unique combination of monoclonal-dye conjugate and polyclonal solid phase antibodies to identify myoglobin in the test samples with a high degree of specificity. As the sample flows through the absorbent device, the labelled antibody-dye conjugate binds to the myoglobin forming an antibody antigen complex. This complex binds to the anti-myoglobin antibody in the positive reaction zone (B) and produces a pink-rose colour band when myoglobin concentration is higher than 100 ng/ml.
In the absence of myoglobin, there is no line in the positive reaction zone. The reaction mixture continues flowing through the absorbent device past the positive reaction zone and control zone. Unbound conjugate binds to the reagents in the control zone (C) producing a pink-rose colour band, demonstrating that the reagents are functioning correctly