BIOPHEN™ Antithrombin 5
BIOPHEN™ Antithrombin 5 (221105) is a Chromogenic Assay for measuring Antithrombin in plasma with an Anti-Xa method. Manufactured by HYPHEN BioMed.
BIOPHEN™ Antithrombin assay is a kinetic method based on the inhibition of FXa, which is at a constant concentration and in excess, by AT in presence of heparin. The remaining FXa is then measured by its amydolitic activity on a FXa specific chromogenic substrate, which releases pNA. The amount of pNA generated is inversely proportional to the AT concentration present in the tested plasma5 . Due to the assay’s insensitivity to heparin, plasmas from patients on heparin therapy may be tested. Heparin + AT ➔[AT Hep.] [AT Hep.] + [Excess FXa] ➔ [FXa-AT-Hep.] + [Remaining FXa] [Remaining FXa] + SXa-11 ➔ Peptide + pNA