Contact Us

Contact Us

Please contact us below with any questions

info@quadratech.co.uk

+44 (0)33 3321 2371

Side Contact
×
Sign up to our newsletter Click Here
quadratech Logo
{{ toggleHam ? 'CLOSE' : 'MENU' }}
Next Day & International Delivery
Competitive Pricing
Over 30 Years Experience
Based In The UK

BIOPHEN™ V-L Plasma Calibrator

X

We're always working hard to give our customers as much information on products and the best price possible. If you need any assistance or would like a quote please contact us and we will be happy to help

BIOPHEN™ V-L Plasma Calibrator (222401) is a kit of Human Plasmas for the calibration of Factor V-Leiden assays by clotting method. Manufactured by HYPHEN BioMed.

Product Brand:
Product Code: 222401
Description

Intended Use

The BIOPHEN™ V-L Plasma Calibrator kit consists of undiluted lyophilized human plasmas, titrated at various concentrations of Factor V-Leiden (FV-L), for the calibration of FV-L assay.

It is titrated and optimized for the assay of FV-L by clotting method.

 

Summary and Explanation

Technical Activated Protein C plays a role of regulator in the coagulation process by specifically inactivating activated Factors V (Va) and VIII (VIIIa), in the presence of co-factors. The resistance phenomenon to activated Protein C (APC) is due in more than 90% of cases to the R506Q mutation of Factor V called “Factor V Leiden”. This mutation in Factor V exon 10 (1691 G → A) substitutes arginine at position 506 by glutamine, prevents this site cleavage by APC.

These calibrators are used to establish the calibration curve for clotting assay of FV-L in plasma (HEMOCLOT™ Quanti. VL).

Clinical FV-L mutation is the most common hereditary thrombophilia risk factor. Its prevalence is about 5% in Caucasian populations. Patients carrying FV-L mutation have an increased risk of venous thrombosis, 3 to 7 fold in heterozygotes and up to 80 fold in homozygotes.

This genetic anomaly can be evidenced by clotting assay in the presence or absence of APC.

 

Reagents

CAL1 Calibrator 1: Undiluted lyophilized human plasma, containing a titrated quantity of FV-L of approximately 10% (plasma presenting APC resistance) (level 1). CAL2 Calibrator 2: Undiluted lyophilized human plasma, containing a titrated quantity of FV-L of approximately 25% (plasma presenting APC resistance) (level 2). CAL3 Calibrator 3: Undiluted lyophilized human plasma, containing a titrated quantity of FV-L of approximately 50% (plasma presenting APC resistance) (level 3).

Each kit contains 3 vials of 0.5 mL for each calibrator (CAL1, CAL2, and CAL3).

Calibrator plasmas contain stabilizing agents.

The calibrator concentrations may vary slightly from one batch to another. For the assay, see the exact values indicated on the flyer provided with the kit used.

Note: The 1:10 dilution of CAL3 in Imidazole buffer allows obtaining the first point of the calibration curve, at about 100% of FV-L (the exact concentration is twice the concentration “C” indicated on the corresponding flyer).

Product Specification
Specification Type
Specification Value
Product Range
Product Code
222401
Product Name
BIOPHEN™ V-L Plasma Calibrator
Product Category
Product Brand
Product Analyte or Application
Product Size
3 x 3 x 0.5ml
Product Storage
2°C to 8°C
Product IVD Registration Status
510(k)
CE-Mark - Professional Use
UKCA
Product Documents

Our Accreditations

Receive Our Latest News
Sign Up To Our Newsletter